The three-dimensional shapes of protein molecules are critical for guiding proper function. Although very detailed static pictures of protein molecules have been produced, macromolecular function is, in many cases, intimately coupled to flexibility and knowledge of molecular motions is thus critical. The goal of this research is to develop new Nuclear Magnetic Resonance Spectroscopy based techniques for relating molecular motions to function. The methodology will be applied to the study of a number of important biological molecules, including the proteasome, the central molecular machine that is a target for anti-cancer and anti-inflammatory drugs.