Cystic fibrosis (CF) is the most common fatal genetic disorder affecting Canadian children. CF lung disease is the major cause of mortality in these patients and is marked by airway obstruction and chronic bacterial infections, particularly by Pseudomonas aeruginosa. Chronic pulmonary infections occur despite excessive airway accumulation of neutrophils, an important immune cell that fights infections, suggesting altered immune responses in CF patients. Recent studies have shown that neutrophils can cast their DNA to form neutrophil extracellular traps (NETs) to entrap microbial pathogens. We hypothesize that chronically adapted P. aeruginosa strains will be more resistant to NET-mediated killing. Using P. aeruginosa strains isolated from CF patients, this study aims to understand the mechanisms P. aeruginosa utilizes to overcome the immune system. The findings of this study will further reveal potential targets for novel therapeutic interventions against CF.