Central venous catheters have been frequently associated with Central line associated bloodstream infections (CLABSI), which are a leading cause of health care associated infection with substantial morbidity and mortality. The source of the CLABSI is usually the lumen of the Central venous catheter (CVC), where microbial organisms, being embedded in a layer of fibrous glycocalyx biofilm, become resistant to conventional antibiotics. In addition to systemic antimicrobial therapy, current management of CLABSI typically involves removal of the culprit catheter and reinsertion of a new CVC at a different site. Attempts have been made to salvage the infected catheter by locking the CVC lumen with a high conCentreation of antibiotics, but the lack of efficacy of the commonly used lock solutions in eradicating the biofilm-embedded organisms has produced disappointing results. However, compelling preliminary data developed by our research team have demonstrated that a highly effective antimicrobial lock solution consisting of minocycline in combination with a chelator ethylenediaminetetraacetic acid (EDTA) in 25% ethanol is highly efficacious in eradicating organisms embedded in biofilm