Breast cancer is the second most common cancer among women in Uganda. Breast cancer risk reduction and screening practices are considered one of the most effective tools for reducing breast cancer mortality. This study aims at assess the breast cancer risk perception, screening and risk reduction behaviors and practices among the first degree female relatives of breast cancer patients in Uganda. The main objective is to assess self-perceived risk of breast cancer, screening and prevention practices of the first degree female relatives of breast cancer patients at the Uganda Cancer Institute. A cross-sectional study employing both quantitative and qualitative methods of data collection. Findings from this study could inform interventions and policies to increase uptake of breast cancer screening services, and promote risk reduction behaviors in this population.